Table 1.
RS activities in leaves of wild-type (Col-0) and RS5 mutants subjected to various abiotic stresses
| 4°C | WD | NaCl | MV | 30°C | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| |
NS |
S |
NS |
S |
NS |
S |
NS |
S |
NS |
S |
| Col-0 |
0.46 ± 0.046 |
1.86 ± 0.138 |
0.55 ± 0.014 |
1.17 ± 0.065 |
0.18 ± 0.005 |
0.34 ± 0.002 |
0.22 ± 0.004 |
0.33 ± 0.006 |
0.24 ± 0.004 |
0.33 ± 0.002 |
|
rs 5-1 |
0.15 ± 0.044 |
0.16 ± 0.028 |
0.16 ± 0.006 |
0.14 ± 0.002 |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
n.d. |
| rs 5-2 | 0.11 ± 0.061 | 0.12 ± 0.025 | 0.17 ± 0.009 | 0.16 ± 0.002 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
Assays were conducted with desalted leaf crude extracts incubated with 100 mM Suc and 10 mM Gol at pH 7.5 for 1 h. NS, non-stressed; S, stressed (as per Materials and Methods); 4°C, cold-stressed; WD, water deficit-stressed; NaCl, salt-stressed; MV, methyl viologen-stressed; 30°C, heat-stressed. Values in bold represent increased RS activities in stressed as compared to non-stressed leaves. Values are the means ± SE of three independent replicates and represent RS activities expressed in nkat g-1 DW.