Figure 2.

Fe concentration in the Fer-NAS-YSL2 lines (Masuda et al.2012). a; The Fe concentrations in T₂ polished rice seeds (Oryza sativa cv. Tsukinohikari). Bars represent the Fe concentrations in polished seeds obtained from individual transgenic or non-transgenic plants. The numbers indicate the line numbers of the independent T₁ lines. The arrows and numbers above the bars show the lines that contained high levels of Fe, which are shown in b for the subsequent generation. b; Fe concentrations in the T₃ polished rice seeds (Oryza sativa cv. Tsukinohikari) harvested from the paddy field. ANOVA with the Tukey–Kramer HSD test was used for each four-block dataset (n = 4). The letters above the bars indicate significant differences (P < 0.05). NT, Non-transgenic rice; AN, OsActin1 promoter–HvNAS1 transgenic rice line No. 8 (Masuda et al. 2009); Fer-NAS-YSL2, transgenic rice lines that carry the OsGlb1 promoter–SoyferH2, OsGluB1 promoter–SoyferH2, OsSUT1 promoter–OsYSL2, OsGlb1 promoter–OsYSL2, and OsActin1 promoter–HvNAS1 (Masuda et al. 2012).