Table 2.
Approaches of Fe biofortification of rice: multi transgenic approaches
| Approach | Introduced genes a | Rice cultivar | Cultivation | Fold increase in Fe concentration compared to non-transgenic rice b . | References |
|---|---|---|---|---|---|
| condition | |||||
| Combination of approaches 1 and 2 | OsGlb pro-Pvferritin | Japonica cv. Taipei 309 | Hydroponic culture | 6 fold (polished seeds) | Wirth et al. 2009 |
| 35S pro-AtNAS1 | |||||
| OsGlb pro-Afphytase | |||||
| Combination of approaches 1, 2 and 3 | OsGluB1 pro-SoyferH2 | Japonica cv. Tsukinohikari | Soil cultivation in greenhouse | 6 fold (polished seeds) | Masuda et al. 2012 |
| OsGlb1 pro-SoyferH2 | |||||
| OsActin1 pro-HvNAS1 | Paddy field | 4.4 fold (polished seeds) | |||
| OsSUT1 pro-OsYSL2 | |||||
| OsGlb1 pro-OsYSL2 | |||||
| OsGluB1 pro-SoyferH2 | Tropical Japonica cv. Paw San Yin (Myanmar High Quality Rice) | Soil cultivation in greenhouse | 3.4 fold (polished seeds) | Aung et al. 2013 | |
| OsGlb1 pro-SoyferH2 | |||||
| OsActin1 pro-HvNAS1 | |||||
| OsSUT1 pro-OsYSL2 | |||||
| OsGlb1 pro-OsYSL2 | |||||
| Combination of approaches 1 and 4 | OsGluB1 pro-SoyferH2 | Japonica cv. Tsukinohikari | Normal soil in greenhouse | 4 fold (polished seeds) | Masuda et al. 2013 |
| OsGlb1 pro-SoyferH2 | |||||
| HvNAS1, HvNAAT-A,-B and IDS3 genome fragments | Calcareous soil in greenhouse | 2.5 fold (polished seeds) |
aThese gene expression cassettes were introduced concomitantly. b.The tissue name written in parentheses is the rice tissue where Fe concentration was increased.