Skip to main content
. Author manuscript; available in PMC: 2014 Jan 2.
Published in final edited form as: DNA Repair (Amst). 2012 Jul 4;11(8):10.1016/j.dnarep.2012.06.002. doi: 10.1016/j.dnarep.2012.06.002

Fig. 1.

Fig. 1

A 60 min treatment with H2O2 or MMS causes persistent mtDNA damage. Cells were plated and the following day were treated for 15 or 60 min with various concentrations of H2O2 or for 60 min with 2 mM MMS in serum-free media. After the treatment, the media was replaced with conditioned media and the cells were allowed to recover for 0 or 8 h. Cells at 0 h recovery were immediately harvested following treatment. mtDNA lesions at 0 and 8 h after a (A) 15-min H2O2 treatment, (B) a 60-min H2O2 treatment or a (C) 60-min MMS treatment. Error bars represent the SEM of n = 4–19, with 2–9 biological experiments and 2–3 replicates per treatment type. H2O2 at 400 μM was not used to treat cells for 60 min. One-sided ANOVA and a Tukey test were used to analyze these data, p < 0.05 (*).