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. Author manuscript; available in PMC: 2014 Jan 2.
Published in final edited form as: DNA Repair (Amst). 2012 Jul 4;11(8):10.1016/j.dnarep.2012.06.002. doi: 10.1016/j.dnarep.2012.06.002

Fig. 2.

Fig. 2

H2O2 and not MMS causes rapid loss of mtDNA. Cells were plated and the following day were treated for 15 or 60 min with various concentrations of H2O2 in serum-free media or for 60 min with 2 mM MMS in serum-free media. After the treatment, the media was replaced with conditioned media and the cells were allowed to recover for 0 or 8 h. Cells at 0 h recovery were immediately harvested following treatment. Fraction of mtDNA copy number remaining at 8 h after a (A) 15-min H2O2 treatment, (B) 60 min H2O2 treatment or a (C) 60-min MMS treatment. Error bars represent the SEM of n = 4–19, with 2–9 biological experiments and 2–3 replicates per treatment type. H2O2 at 400 μM was not used to treat cells for 60 min. One-sided ANOVA and a Tukey test were used to analyze these data, p < 0.05 (*). There is a significant difference in the mtDNA copy number at 8 h when comparing cells treated with 200 μM H2O2 for 15 min to cells treated with 200 μM H2O2 for 60 min.