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. Author manuscript; available in PMC: 2014 Jan 2.
Published in final edited form as: DNA Repair (Amst). 2012 Jul 4;11(8):10.1016/j.dnarep.2012.06.002. doi: 10.1016/j.dnarep.2012.06.002

Fig. 4.

Fig. 4

H2O2 and MMS affect mitochondrial complex levels differently. Cells were plated and the following day were treated for 15 or 60 min with 200 μM H2O2 or for 60 min with 2 mM MMS in serum-free media. After the treatment, the media was replaced with conditioned media and the cells were allowed to recover for 8 h. Cells at 8 h recovery were harvested and subjected to a multiplex assay detecting complex I, complex III, and complex V levels. Histogram of complex I, III, and V levels after H2O2 or MMS treatment. Three individual experiments were performed, with 1–2 replicates per treatment type. Light gray bar, control; green striped bar, 2 mM MMS for 60 min; black bar, 200 μM H2O2 for 60 min. Error bars represent SD, n = 5–6. A one-way ANOVA was used to analyze these data, p < 0.05 (*). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)