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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Prostate. 2013 Jul 3;73(14):10.1002/pros.22706. doi: 10.1002/pros.22706

Figure 3. A fraction of TMEFF2 localizes to the cytoskeleton in 22Rv1/sh_SARDH cells.

Figure 3

A: Lysates from 22Rv1 cells expressing sh_RNA to SARDH or sh_scramble as a control were subjected to fractionation. Subcellular fractions were analyzed by western blot using an antibody against TMEFF2. Note that localization of TMEFF2 changes in response to reduced levels of SARDH. B: Lysates from 22Rv1 cells expressing sh_RNA to SARDH, TMEFF2 or sh_scramble were prepared in RIPA buffer containing Triton X-100, fractionated via high speed centrifugation into supernatant and pellet fractions and analyzed by western blot analysis. C: Immunofluorescence staining of 22Rv1/sh_SARDH cells. Cells were fixed and stained with anti-TMEFF2 (green) and phalloidin to detect actin (red). Colocalization of TMEFF2 and actin is illustrated by yellow signal.