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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Prostate. 2013 Jul 3;73(14):10.1002/pros.22706. doi: 10.1002/pros.22706

Figure 4. Reducing the levels of TMEFF2 and/or SARDH similarly affects cellular invasion.

Figure 4

A & B: The effect of reducing the levels of TMEFF2 or SARDH using sh_RNA (A) or si_RNA (B) was analyzed using Boyden chambers. Cells adhering to the bottom of the membrane were fixed, stained with crystal violet and photographed. Representative images (left) of at least three independent experiments with multiple replicates, and quantification of the results (right) are shown. C: The effect of reducing the levels of TMEFF2 and SARDH simultaneously using sh_RNA to silence SARDH and si_RNA to silence TMEFF2 was analyzed as described for A and B. 22Rv1 cells expressing the scramble sh_RNA scramble or a non-silencing (NS) si_RNA were used as controls. (*) p < 0.05.