Table 3.
Strains and plasmids used in this study
| Strain/plasmid | Description | Reference a |
|---|---|---|
| CCTCC M94055 |
An industrial ethanol-producing S. cerevisiae strain; MATa/α |
CCTCC |
| CIBTS0525 |
S. cerevisiae CCTCC M94055 derivative; arg1::pCpA1/G-XI; ty1::pYIE2-Ty-XI; δ::loxP-zeo-loxP-ADH1p-XKS1-XKS1t-TPI1p-TAL1-TAL1t-PGK1p-RPE1-RPE1t-FBA1p-TKL1-TKL1t-PDC1p-RKI1-RKI1t |
This study |
| CIBTS0555 |
A single-colony isolate of CIBTS0525 after adaptive evolution |
This study |
| CIBTS0573 |
CIBTS0525 derivative; gre3::pYIE2-GXF1 |
This study |
| CIBTS0735 |
A single-colony isolate of CIBTS0573 after adaptive evolution |
This study |
| pCpA1/G-XI |
Carrying TPI1p-XYLA-CYC1t, used to integrate a copy of Piromyces XYLA at the ARG1 locus |
This study |
| pYIE2-Ty-XI |
Carrying TPI1p-XYLA-CYC1t, used to integrate a copy of Piromyces XYLA at the Ty1 locus |
This study |
| pSH47-hph |
pSH47 encoding a hygromycin-resistant protein |
This study |
| pYIE2-GXF1 | Carrying TPI1p-GXF1-TPI1t, used to integrate Candida intermidia GXF1 at the GRE3 locus | This study |
aCCTCC, China Center for Type Culture Collection.