Table 2.
Primers used for real-time qPCR
| Target group | Primer | Sequence (5′-3′) | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
|
Enterobacteriaceae |
Eco1457F |
CATTGACGTTACCCGCAGAAGAAGC |
63 |
[18] |
| |
Eco1652R |
CTCTACGAGACTCAAGCTTGC |
|
|
|
Veillonella |
Vei343F |
A(C/T)CAACCTGCCCTTCAGA |
62 |
[18] |
| |
Vei343F |
CGTCCCGATTAACAGAGCTT |
|
|
|
Bacteroides |
Bac566F |
GGGTTTAAAGGGAGCGTAGG |
53 |
[19] |
| |
Bac692R |
CTACACCACGAATTCCGCCT |
|
|
|
Clostridium |
CI-F |
TACCHRAGGAGGAAGCCAC |
54 |
[20] |
| CI-R | GTTCTTCCTAATCTCTACGCAT |