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. 2004 May;72(5):2564–2573. doi: 10.1128/IAI.72.5.2564-2573.2004

FIG. 5.

FIG. 5.

C3 binds to M. tuberculosis proteins. M. tuberculosis bacilli (2.5 × 107) were incubated with NHS in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of 10 mM EDTA, washed, resolved by SDS-PAGE under reducing conditions, and transferred to nitrocellulose. The lanes were divided and then incubated in anti-C3 antibody (lanes 1 and 2), or anti-M. tuberculosis antiserum (lanes 3 and 4). The results shown are representative of three independent experiments. The molecular mass markers are shown on the left.