FIG. 7.

C3 protein is detected in BAL fluid. Purified C3 protein (200, 100, 50, or 25 ng in lanes 1 to 4, respectively), 20 μl of BAL fluid (lane 5), or 20 μl of twofold serial dilutions of cBAL fluid (lanes 6 to 8) was resolved by SDS-PAGE under reducing conditions and subjected to Western blotting as described in the legend of Fig. 1. The molecular mass indicators are shown on the right. The positions of C3α and C3β are shown on the left. The results shown are representative of three independent experiments.