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. 2004 May;72(5):2889–2898. doi: 10.1128/IAI.72.5.2889-2898.2004

FIG. 3.

FIG. 3.

Quantification of fluorescence microscopy data using acridine orange and ethidium bromide staining. (A) Infection with H. pylori at MOI of 10:1, 30:1, 50:1, and 100:1 for 24 h resulted in a dose-dependent induction of apoptosis, with increased levels of apoptosis observed at MOI of 50:1 and 100:1 compared with uninfected controls (23.1% ± 8.4% and 27.9% ± 12.9%, respectively, versus 9.9% ± 5.8%; ANOVA-Bonferroni multiple-comparison test; *P < 0.004; n = 5). (B) RAW 264.7 cells infected with H. pylori (solid bars) at an MOI of 50:1 for 6, 24, or 48 h showed a time-dependent increase in apoptosis at 24 h compared with time-matched uninfected controls (open bars) (13.5% ± 2.9% versus 4.0% ± 1.0%; unpaired t test; *, P < 0.0004; n = 4). The results are expressed as mean percentages of apoptotic cells per 500 cells enumerated, and the error bars represent standard errors of the mean.