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. 2004 May;72(5):2889–2898. doi: 10.1128/IAI.72.5.2889-2898.2004

FIG. 7.

FIG. 7.

Infection of RAW 264.7 cells with H. pylori leads to a time-dependent decrease in the expression of uncleaved Bid protein. (A) Whole-cell protein extracts from RAW 264.7 cells analyzed by immunoblotting. (Top) Uninfected RAW 264.7 cells (−) expressed both uncleaved (22-kDa) and cleaved (15-kDa) Bid protein. In contrast, uncleaved Bid protein expression decreased following infection with H. pylori (MOI, 100:1) (+) beginning at 6 h. (Bottom) Actin levels were assayed to monitor protein loading between samples (n = 3). (B) Densitometry corresponding to immunoblots from three separate experiments reflects decreased uncleaved Bid protein expression in H. pylori-infected samples (solid bars) when normalized to actin levels to control for protein loading (open bars) over time (*, P < 0.05; **, P < 0.001; ANOVA; n = 3). Error bars, standard errors.