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. 2013 Dec 17;12:126. doi: 10.1186/1475-2859-12-126

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Copyright © 2013 Kirm et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Phenotypes of S. erythraea mutants with inactivated or in trans - overexpressed SACE_5599 on ABSM4 agar plates containing apramycin: A) NRRL23338/pSET152; B) ABE1441/pSET152; C) ABE1441/ΔSACE_5599 complemented with SACE_5599 (pABE110; pABE112); D) ABE1441/ΔSACE_5599 (pABE110); E) ABE1441/pSET152 + SACE_5599 (pABE104) F) NRRL23338/pSET152 + SACE_5599 (pABE104). Construction of plasmid constructs is presented in Table 1. Equivalent result was observed when plates without apramycin were used (not shown).