Table 1.
Strains and plasmids used in this study
| Vector/strain name | Description | Promoter | Gene | Reference |
|---|---|---|---|---|
|
S.erythraea |
WT- NRRL23338 |
/ |
/ |
[31,32] |
|
S.erythraea |
ABE1441 |
/ |
/ |
Acies Bio |
| pABE53 |
ɸC31, apramycin |
PermE* without RBS |
SACE_5599 (184 aa) |
This work |
| pABE101 |
ɸC31, apramycin |
PermE* with RBS |
SACE_5599 (184 aa) |
This work |
| pABE102 |
ɸC31, apramycin |
PermE* without RBS |
SACE_5599 (184 aa) HA-tag |
This work |
| pABE103 |
ɸC31, apramycin |
PermE* with RBS |
SACE_5599 (184 aa) HA-tag |
This work |
| pABE104 |
ɸC31, apramycin |
PermE* without RBS |
SACE_5599 (219 aa) |
This work |
| pABE105 |
ɸC31, apramycin |
PermE* with RBS |
SACE_5599 (219 aa) |
This work |
| pABE106 |
ɸC31, apramycin |
PermE* without RBS |
SACE_5599 (219 aa ) HA-tag |
This work |
| pABE107 |
ɸC31, apramycin |
PermE* with RBS |
SACE_5599 (219 aa) HA-tag |
This work |
| pABE110 |
Suicide, apramycin |
/ |
344 bp long part of the SACE_5599 gene for disruption |
This work |
| pABE21 |
ɸC31, apramycin |
PermE* without RBS |
bldD gene |
This work |
| pABE112 | ɸC31, thiostrepton | PermE* without RBS | SACE_5599 (219 aa ) HA-tag | This work |
Site-specific integration with bacteriophage ɸC31 integrase is indicated for pSET152-derived plasmids; “/” refers to “none or not applicable”.