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. 2013 Dec 17;9:255. doi: 10.1186/1746-6148-9-255

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Copyright © 2013 Gargiulo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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LDPI scan technique. (A) Animal positioning in sternal recumbency on a light-absorbing pad, with the hind plantar surfaces symmetrical and perpendicular to the laser beam. (B) LDPI image post-processing and measurement standardized protocol: the mean intensity of the Doppler signal was registered in ROI encompassing the hind paws and expressed as numerical value normalized for their area (perfusion color scale 0–5 volts).