Table 1.
Fold-change (at P < 0.05) observed in secreted cytokines, chemokines and growth factors from isolated endometrial cells following administration of recombinant hCG (100 IU/ml) in three culture groups
| Name |
Groups (Cell type) |
||
|---|---|---|---|
|
1 |
2 |
3 |
|
| (Epithelial) | (Stromal) | (Mixed) | |
|
CCL2
1
|
(ι) |
- |
- |
|
CCL3
2
|
- |
- |
(+)8.0 |
|
CCL4
|
(+)1.7 |
(+)1.55 |
(+)8.0 |
|
CCL5
2
|
(+)1.8 |
- |
(+)2.8 |
|
CCL7 |
(-)1.7 |
- |
- |
|
CXCL1 |
(-)6.5 |
- |
- |
|
CXCL9 |
- |
- |
(-)1.7 |
|
CXCL10
3
|
- |
- |
(+)2.7 |
|
CXCL12 |
(-)2.1 |
- |
- |
|
FGF2
3
|
- |
(+)1.5 |
(+)2.3 |
|
GCSF
2
|
- |
- |
(+)1.4 |
|
GMCSF
3
|
- |
(+)1.5 |
(+)2.7 |
|
HGF |
(-)1.6 |
- |
(-)1.7 |
|
IFNG
2
|
- |
(+)1.5 |
(+)2.0 |
|
IL-1b
1
|
- |
(+)2.7 |
(+)3.3 |
|
IL-6
2
|
(+)2.1 |
(-)1.6 |
(-)2.3 |
|
IL-8 |
- |
- |
(-)2.6 |
|
IL-12p40 |
(-)1.5 |
- |
- |
|
IL-12p70
|
- |
- |
(ι) |
|
IL-13
2
|
- |
- |
(+)1.8 |
|
IL-16
1
|
- |
(-)2.7 |
(-)1.8 |
|
IL-17
2
|
- |
(+)2.1 |
- |
|
LIF
4
|
(+)1.5 |
|
(+)1.7 |
|
MCSF |
(-)1.5 |
- |
(-)1.6 |
|
MIF |
(+)1.7 |
(-)1.5 |
(-)2.4 |
|
PDGFBB
1
|
- |
- |
(ι) |
|
SCGF |
(-)3.5 |
- |
- |
|
TNF
2
|
- |
- |
(+)1.7 |
|
TRAIL |
(+)1.5 |
- |
(-)1.6 |
| VEGF 1 | - | (+)1.9 | (+)2.5 |
_, no change. (ι), infinitesimally increased because of non-detectable amount under the basal condition. (), up-regulated. (~) down-regulation. The cytokines which were subjected to Western blot analysis are shown in bold, and the ones which were subjected to quantitative real time RT-PCR for estimation of relative abundance of transcripts are shown in italics.
1did not show significant concordance between immunopositive protein concentration in medium and relative abundance of transcripts for all cell culture groups (groups 1–3).
2showed significant (P < 0.05) concordance between immunopositive protein concentration in medium and relative abundance of transcripts for all cell culture groups (groups 1–3).
3showed significant (P < 0.05) concordance between immunopositive protein concentration in medium and relative abundance of transcripts for epithelial cells (group 1) and mixed cells (group 3), but not for stromal cells (group 2).
4showed significant (P < 0.05) concordance between immunopositive protein concentration in medium and relative abundance of transcripts for stromal cells (group 2) and mixed cells (group 3), but not for epithelial cells (group 1).
5no concordance was seen between profiles from multiplex based immunoassay and from densitometric analysis of Western immunoblot.