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. 2013 Dec 17;10:355. doi: 10.1186/1743-422X-10-355

Table 1.

Amplification and sequencing primers for HCV NS5A and NS5B in genotypes 1a and 1b

Gene Primer name Primer sequence
GT1a* NS5A
HCV-1a NS5A Fwd out
GACATCTGGGACTGGATATGYGA
 
HCV-1a NS5A Rew out
GTCCAGGWRTARGACATYGAGCA
 
HCV-1a NS5A Fwd inn
GATATGYGAGGTGYTGAGCGA
 
HCV-1a NS5A Rew inn
GAGCARCACACGACRTCYTC
 
HCV-1a NS5A Rew Seq
AAGGAGTCCARRATCACCAC
 
HCV-1a NS5B Fwd out
GGAGCCKGGRGATCCRGATCTYAGC
GT1a* NS5B
HCV-1a NS5B Rew out
GTTGGGGAGGAGGTAGATGCCTA
 
HCV-1a NS5B Fwd inn
GAYGTCGTGTGCTGCTCRATGTC
 
HCV-1a NS5B Rew inn
GAGACACGCTGTGATAAATGTCTCCC
 
HCV-1a NS5B Fwd Seq
TCGTAAGCCAGCTCGYCTCATCG
 
HCV-1a NS5B Rew Seq
CCTATTGATTTCACCTGGAGAG
 
HCV-1b NS5A Fwd out
GGATYAAYGARGACTGYTCYAC
 
HCV-1b NS5A Rew out
GACCARGACCCGTCRCTGAGRT
 
HCV-1b NS5A Fwd inn
GGGAYTGGATATGCACGGT
GT1b § NS5A
HCV-1b NS5A Rew inn
GGCATGGAGGARTAYGAC
 
HCV-1b NS5A Fwd Seq
ARTTGTCTGCGCCTTCYYTGAAGG
 
HCV-1b NS5B Fwd out
CGYTGAGTCRTAYTCCTCCATGC
 
HCV-1b NS5B Rew out
GGGCRCGAGACASGCTGTGATA
 
HCV-1b NS5B Fwd inn
CTCAGYGACGGGTCYTGGTC
GT1b § NS5B
HCV-1b NS5B Rew inn
GCTGTGATATATGTCTCCCC
 
HCV-1b NS5B Fwd Seq2
TGGGRGTHCGYGTRTGCGAG
  HCV-1b NS5B Rew Seq3 AGCATYGTGCAGTCCYGGAGC

*Genotype 1a; § Genotype 1b.