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Percentage of CD4-T-cell migration to purified wild-type pneumolysin and the W433>F mutant. Migration to purified pneumolysin is represented as dotted columns, and migration to the W433>F mutant is represented as lined columns (each experiment was repeated three times, with each sample within one experiment done in triplicate). Data represent the mean of all experiments, with error bars indicating the standard error of mean.
