Figure 8.

Severing both of the elongated processes of ALA abolished its responses to picking-touch stimuli that were positioned posterior to the cut. (A) A representative image of a single ALA process expressing GCaMP3 before (left) and after (right) it was severed. (B-C) GCaMP3 fluorescence levels in ALA neurons before and after a picking-touch stimulus was administered posterior to the cut in operated animals (B) and in mock-operated controls (C). Mean ± s.e.m, N_operated=8 and N_mock=10 animals. (D) Same as (B), but for stimuli that were administered anterior to the cut, i.e., between the cut and the ALA cell body. Mean ± s.e.m, N_operated=11 animals. Dashed lines in panels (C-D) are provided as a guide to the eye.