Figure 1.

Histology of the radial artery samples. (a) Morphology of the routinely (HE) stained artery showing intimal thickening (I) and no mineral content. I: intima; M: media; A: adventitia. (b)–(d) Serial sections of the entire sample of the arterial wall demonstrating very advanced calcification (grade 4) stained with HE (b), von Kossa (c), and alizarin red (d). (e) Large granular calcifications (grade 3) localized on both sides of the media (alizarin red staining). (f) Small (grade 2) calcifications in the media (alizarin red staining). *Lumen of the vessel.