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Time-dependent uptake of [14C]chenodeoxycholic acid by Denmark::pGH1 and Denmark::mmaA3. Permeability was measured as uptake of the marker [14C]chenodeoxycholic acid over time. At each time point, aliquots of Denmark::pGH1 (•) and Denmark::mmaA3 (□) cell suspensions were applied to cellulose filters by vacuum filtration and washed, and the incorporated radioactivity was quantified by liquid scintillation counting. Data shown are from an experiment repeated twice with similar results.
