Fig. 7.

Rac1 is required for basal membrane protrusions in the distal salivary gland. Distal salivary gland cells expressing Actin-GFP in wild-type embryos (A) and mew^M6 homozygous embryos expressing Rac1^V12 in the gland (D) extend basal membrane protrusions (A and D, arrowheads). Distal salivary glands expressing Rac1^N17 and Actin-GFP (B) or glands of mew^M6 homozygous embryos expressing Actin-GFP (C) do not extend basal membrane protrusions (B and C, arrowheads). Pie-charts show percentage of glands with indicated number of basal membrane protrusions per gland in wild-type glands expressing actin-GFP in the gland, glands co-expressing Rac1^N17and actin-GFP, mew^M6 mutant glands expressing actin-GFP in the gland and mew^M6 mutant glands co-expressing Rac1^V12 and actin-GFP (E). In wild-type embryos (F) and mew^M6 heterozygous embryos (H), distal gland cells are elongated (F and H, arrowheads) whereas in rac1^J11rac2^Δ (G) and mew^M6 (I) mutant embryos, distal gland cells are not elongated (G and I, arrowheads). Graph shows apical-basal length of distal gland cells in mew^M6 heterozygous embryos, rac1^J11rac2^Δ and mew^M6 mutant embryos and embryos expressing Rac1^N17 in the gland (J). Graph shows extent of salivary gland migration at stage 14 in rac1^J11rac2^Δ heterozygous and homozygous embryos and wild-type and rac1^J11rac2^Δ embryos expressing Rac1^F37A or Rac1^Y40C (K). Panels A–D are live-images of embryos expressing Actin-GFP specifically in the salivary gland with fkh-GAL4. Embryos in panels F and G were stained for E-cad (red), FasIII (white) and β-gal (not shown). Embryos in H and I were stained for F-actin (green). All embryos shown are at stage 13. Scale bar represents 10 μm.