Table 2.
Primer sequences used for RT qPCR analysis.
| Primer sequences used qRT-PCR analysis (mouse) | ||
|---|---|---|
| GENE | FORWARD 5′-3′ | REVERSE 5′-3′ |
| Gapdh | TGTGATGGGTGTGAACCACGAGAA | CATGAGCCCTTCCACAATGCCAA A |
| Sox17 | ACCTACACTTACGCTCCAGTC | GCCGTAGTACAGGTGCAGAG |
| Kdr | AGGCCC ATTGAGTCCAACTACACA | ATGTCTGCATGGTCCCATACTGG T |
| Nkx2-5 | TGGGTCTCAATGCCTATGGCTACA | GACGCCAAAGTTCACGAAGTTG CT |
| Gata4 | CACGCTGTGGCGTCGTAAT | CTGGTTTGAATCCCCTCCTTC |
| Tbx5 | TGGCTGAAGTTCCACGAAGTG | TTTGGGATTAAGGCCAGTCAC |
| Tnnt2 | TCACAACCTGGAGGCTGAGAAGTT | TCATCTATTTCCAACGCCCGGTG A |
| Myh6 | AGCTGACAGGGGCCATCAT | ACATACTCGTTCCCCACCTTC |
| Myh 7 | TGCCAATGACGACCTGAAGGAGA A |
TCTTCTGGTTGATGAGGCTGGTG T |
| Myl2 | AGATGCTGACCACACAAGCAGAG A |
TCCGTGGGTAATGATGTGGACC AA |
| Myl7 | AAGGGAAGGGTCCCATCAACTTCA | AACTTGTCTGCCTGGGTCATGAG A |
| Neurod | GACGGGGTCCCAAAAAGAAAA | GCCAAGCGCAGTGTCTCTATT |
| Pecam | TCTATGACCTCGCCCTCCACAAA | GAACGGTGTCTTCAGGTTGGTAT TTCA |
| Cdh5 | TGGAGAAGTGGCATCAGTCAACAG | TCTACAATCCCTTGCAGTGTGAG |
| Gata1 | TGTCCTCACCATCAGATTCCA | TCCCTCCATACTGTTGAGCAG |
| Myod1 | CCACGACCACCTCTCAGAAC | GACAGGACAGTATGCAGTGGA |
| Ddr2 | ATGATCCCGATTCCCAGAATGC | CATATTTGGCAGCCGTGGATT |
| Acta2 | CATGTACGTCGCCATTCAAGC | TTGATGTCTCGCACAATTTCTCT |
| Primer sequences used qRT-PCR analysis (Human) | ||
|---|---|---|
| GENE | FORWARD 5′-3′ | REVERSE 5′-3′ |
| GAPDH | AGGTGGTCTCCTCTGACTTCAACA | GACAAAGTGGTCGTTGAGGGCA AT |
| OCT4 | ATGCATTCAAACTGAGGTGCCTGC | CCACCCTTTGTGTTCCCAATTCCT |
| T | TGTCCCAGGTGGCTTACAGATGAA | GGTGTGCCAAAGTTGCCAATACA C |
| KDR | ACTTTGGAAGACAGAACCAAATTAS TCTC |
TGGGCACCATTCCACCA |
| MESP1 | AGCCCAAGTGACAAGGGACAACT | AAGGAACCACTTCGAAGGTGCT GA |
| NKX2-5 | ACCTCAACAGCTCCCTGACTC | ATAATCGCCGCCACAAACTCTCC |
| TBX5 | ACAAAGTGAAGGTGACGGGC | GGGGACCACGGGATATTCTT |
| TNNT2 | TTC ACC AAA GAT CTG CTC CTC GCT |
TTATTACTGGTGTGGAGTGGGTG TGG |
| MYH6 | TCA GCT GGA GGC CAA AAG TAA AGG A |
TTCTTGAGCTCTGAGCACTCGTC T |
| MYH7 | TCGTGCCTGATGACAAACAGGAGT | ATACTCGGTCTCGGCAGTGACTT T |
| MYL2 | TGTCCCTACCTTGTCTGTTAGCCA | ATTGGAACATGGCCTCTGGATGG A |
| MYL7 | ACATCATCACCCATGGAGACGAGA | GCAACAGAGTTTATTGAGGTGCC C |
|
NEURO
D |
TCCCATGTCTTCCACGTTAAGCCT | CATCAAAGGAAGGGCTGGTGCA AT |
|
PECAM
1 |
TTCCTGACAGTGTCTTGAGTGGGT | TTTGGCTAGGCGTGGTTCTCATC T |
| ACTA2 | AATACTCTGTCTGGATCGGTGGCT | ACGAGTCAGAGCTTTGGCTAGG AA |
| MYH11 | GGTCACGGTTGGGAAAGATGA | GGGCAGGTGTTTATAGGGGTT |
| MYOD1 | CGCCATCCGCTATATCGAGG | CTGTAGTCCATCATGCCGTCG |
| DDR2 | ACCAGCCATTTGTCCTGACTCTGT | ATCACTCGTCGCCTTGTTGAAGG A |
| THY1 | ATACCAGCAGTTCACCCATCCAGT | ATTTGCTGGTGAAGTTGGTTCGG G |
| ACTA2 | AATACTCTGTCTGGATCGGTGGCT | ACGAGTCAGAGCTTTGGCTAGG AA |