Table 2.

Autocrine loop induced by EREG in U87 cells

	Division time in days (R value)
	U87Ctrl	U87dn
no treatment	2.73 (0.99)	5.08 (1.00)
EREG	2.20 (1.00)	2.86 (0.99)
EREG/Erbitux®	3.08 (1.00)	4.50 (1.00)
anti-EREG	3.12 (1.00)	4.76 (0.99)

Cells were plated at 10 000 cells/cm² in 24-well plates and grown for four successive passages in serum-free condition in the presence or absence of 10 ng/ml EREG, with or without antibodies anti-ErbB1 (Erbitux®, 200 μg/ml) or anti-EREG (5 μg/ml). Cells were counted at each passage and division times were presented as best slopes obtained after four passages (29 days of growth) and from a series of triplicate experiments. Regression lines include the origin (R, correlation coefficient). Control mouse monoclonal antibody (isotype IgG1) had no significant effect.