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. 2004 May;72(5):2915–2921. doi: 10.1128/IAI.72.5.2915-2921.2004

FIG. 1.

FIG. 1.

Southern blot analyses to determine the presence of episomal and integrated forms of AS-TgCPB. Genomic DNA from the RH wild type (RH), the AS-TgCPB strain (AS-TgCPB), and the antisense vector control (AS-Control) were digested with either SpeI or HindIII and probed for the TgCPB cDNA insert. The cDNA probe cross-reacted with the genomic DNA of all three cell lines. As expected, a 3.4-kb fragment of the AS-TgCPB-ribozyme antisense insert was detected in SpeI-digested AS-TgCPB clones but not in the RH wild type or the control. The intensity and size of the HindIII-digested genomic DNA indicates that plasmid pminHXGPRT-AsTgCP1-RZ (8.3 kb) was integrated into the parasite genome.