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Northern blot analysis of steady-state levels of RNA following transfection with the antisense plasmid. Total RNA (5 μg/lane) was extracted from T. gondii strain RH tachyzoites (wild type), an antisense vector control preparation, and an AS-TgCPB preparation. The Northern blots were hybridized with a toxopain-1 probe (left panel) and with the SAG1 probe (right panel).
