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Western blot analysis of TgCPB levels following antisense inhibition. Total soluble tachyzoite lysates (5.25 μg) were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred to nitrocellulose, and probed with monoclonal antibodies 1.7, 1.15, and 1.17. Immunolabeled proteins were visualized with an ECL kit. The results for tachyzoites transfected with a control vector or pAS-TgCPB are shown.
