FIG. 5.

Inhibition of intracellular multiplication by AS-TgCPB. HFF monolayers were infected with tachyzoites for 2 h, and all free parasites were then removed and replaced with fresh medium. Cultures were incubated for 24 h and fixed, and then the numbers of parasites per vacuole were determined. The values are means ± standard errors from at least three separate experiments. Expression of AS-TgCPB significantly reduced intracellular multiplication of tachyzoites (solid bars) compared to multiplication of the antisense vector control (open bars). The P value was <0.01 as determined by Student's t test.