FIG. 9.
Expression of antisense toxopain-1 and treatment with a hydrazide proteinase inhibitor limit toxoplasma infection in the chicken embryo. (A) Twelve-day-old chicken embryos were injected with 104 tachyzoites expressing the control vector or antisense toxopain-1, and the brains and livers were harvested on day 15. Total DNA was extracted, and the numbers of copies of SAG1 present in the organs were determined by real-time PCR and were extrapolated to the number of toxoplasma cells present based on the standard curve (Fig. 8). (B) RH tachyzoites (104 cells) were preincubated in medium alone or in medium containing the hydrazide inhibitor ZLIII115. The organs were harvested on day 18 and processed as described above. The results of a representative experiment (one of four experiments) are shown. The y axes of the two panels are different because of the higher number of tachyzoites in the liver.