FIG. 1.

Caspase-8 activation in monocytes after phagocytosis of S. aureus. Ac-IETD-AFC was added to monocyte lysates prepared at various times after exposure to bacteria (MO+S.a). Control monocytes without bacteria (MO) were also sampled at the same time points. Ratios of fluorescence signals obtained in samples with S. aureus to those without S. aureus at the indicated time points are shown. Results obtained 15 min after exposure to bacteria are means ± standard deviations of five measurements from independent experiments; results for other time points are means ± standard deviations of measurements from three independent experiments.