Table 4.
Putative secreted proteins identified in the sialotranscriptome of Psorophora albipes and confirmed by our proteomic studies
| Description | Protein name | Fraction → Number of Tryptic peptides* |
|---|---|
| 5′ nucleotidase/apyrase |
Psor-13556|F11→8, Psor-17515|F11→8, Psor-22761|F11→8, Psor-17516|F11→7, Psor-12600|F11→5, Psor-17320|F11→4 |
| Adenosine deaminase |
Psor-13638|F11→163 |
| Endonuclease |
Psor-34082|F12→18, Psor-34081|F12→18, Psor-34084|F12→8 |
| Glycosidases |
Psor-12511|F9→84 |
| Serpin |
Psor-18383|F12→594, Psor-18379|F12→562, Psor-18398|F12→504, Psor-18380|F12→473, Psor-20123|F12→263, Psor-20135|F12→239, Psor-18402|F12→207, Psor-20121|F12→159, Psor-18262|F12→151, Psor-12755|F12→19 |
| Lysozyme |
Psor-13510|F19→7, Psor-14008|F19→7, Psor-12935|F19→7, Psor-29999|F19→7 |
| gSG5 mucin |
Psor-13808|F7→34, Psor-12515|F7→29, Psor-13816|F7→24, Psor-21012|F7→23, Psor-12520|F7→16, Psor-16372|F14→3 |
|
Aedes-specific mucin |
Psor-24903|F14→8 |
| Long-D7 mosquito |
Psor-34191|F15→29, Psor-34198|F15→28, Psor-34194|F15→28, Psor-34190|F15→22, Psor-34202|F15→17, Psor-32651|F15→9, Psor-16545|F14→26, Psor-14130|F14→22, Psor-27735|F14→22, Psor-22249|F14→22, Psor-21941|F14→10, Psor-14244|F14→5, Psor-22962|F14→5, Psor-16516|F14→4, Psor-16517|F14→3, Psor-5239|F14→2 |
| Short-D7 Culicine |
Psor-24290|F19→11, Psor-14799|F19→8, Psor-24280|F19→8, Psor-14821|F19→8, Psor-24282|F19→8, Psor-24283|F19→7, Psor-11438|F19→5, Psor-33959|F19→3 |
| Antigen-5 |
Psor-12020|F16→22, Psor-14194|F16→5, Psor-12762|F15→7, Psor-17302|F15→3 |
| 30-kDa/Aegyptin family |
Psor-20191|F15→39, Psor-18075|F14→19, Psor-18076|F14→18, Psor-18880|F14→10, Psor-19455|F14→7 |
| Basic tail mosquito |
Psor-13880|F19→3 |
|
Aedes 62-kDa |
Psor-23962|F18→7, Psor-23027|F12→4, Psor-18121|F12→2, Psor-15772|F12→2, Psor-15774|F12→2 |
| 9.7-kDa family |
Psor-19686|F19→5, Psor-19685|F19→4, Psor-19523|F19→3, Psor-12072|F19→3 |
| Hyp8.2 Culicine |
Psor-31485|F19→2, Psor-31484|F19→2, Psor-31419|F19→2 |
| 30.5-kDa |
Psor-14979|F15→4 |
| 23.5-kDa |
Psor-16925|F17→2 |
| 34-kDa Aedes |
Psor-15738|F14→2 |
| Psor-4 kDa ultrashort-D7 | Psor-9075|F19→2 |
*Summary in the form of F11 →18∣ F12 →18∣ F13→2∣ indicates that 18 fragments were found in Fraction 11, while 18 and 2 peptides were found in fractions 12 and 13, respectively. This summary included protein identification only when two or more peptide matches to the protein were obtained from the same gel slice.