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. 2013 Dec 13;12:448. doi: 10.1186/1475-2875-12-448

Figure 4.

Figure 4

Expression of circumsporozoite protein gene in E. coli. (A) Purification of recombinant circumsporozoite protein with Ni-NTA agarose affinity chromatography. Lane M, Molecular weight protein marker; lane 1, induced E. coli DH5α cell lysate with IPTG; lane 2, flow-through; lane 3, wash; lane 4, elute. (B) Western blot analysis of recombinant circumsporozoite protein. M, Molecular weight protein marker; lanes 1–3, patients with malaria.