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Growth kinetics of HpuA+B+ DNM140 with Hb and HbHp complexes. Meningococci expressing wild-type HpuAB were grown overnight on Fe-depleted CDM0 plates before being used to inoculate CDM0 broth cultures supplemented with Hb, type 1-1 HbHp, type 2-2 HbHp, or type 2-1 HbHp. Control cultures grown in Fe-replete (CDM100) and Fe-depleted (CDM0) media were included. The data presented are the averaged results of two independent experiments.
