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Energy-dependent dissociation of Hb and HbHp from HpuAB. DNM140, DNM146, and CCCP-treated DNM140 cells were equilibrated with 200 nM FEX-labeled Hb (A) or type 1-1 HbHp (B). Then, 20-fold excess unlabeled competitor was added at various time points and the amount of labeled ligand remaining bound to cells over time was determined. Control samples to which no competitor was added defined 100% binding. Each strain was tested twice in duplicate for each ligand (total of four data sets).
