TABLE 2.
Summary of HpuAB binding kinetics of Hb, apo-Hp, and HbHpa
| Ligand | DNM140 (wild type)
|
DNM69 (HpuA−B+)
|
||
|---|---|---|---|---|
| Kd (nM) | Bmax (AU) | Kd (nM) | Bmaxb (AU) | |
| Hb | 555 | 42 | 409 | 9 |
| 1-1 Hp | 4,249 | 29 | >5,000 | ++ |
| 2-2 Hp | 808 | 26 | >5,000 | ++ |
| 2-1 Hp | 486 | 24 | >5,000 | ++ |
| 1-1 HbHp | 119 | 23 | >5,000 | ++ |
| 2-2 HbHp | 84 | 21 | >5,000 | ++ |
| 2-1 HbHp | 39 | 20 | >5,000 | + |
a
No binding to DNM68 was detected, and only very low binding of types 2-2 and 1-1 HbHp to DNM143 was detected.
b
+ and ++ indicate relative binding (nonsaturable) of ligands to DNM69.