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Role of TonB and an intact PMF in binding and dissociation kinetics
| Ligand | DNM140 (wild type)
|
DNM146 (TonB−)
|
DNM140 + CCCP
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| Kd (nM) | Bmax (AU) | t1/2 (min) | Kd (nM) | Bmax (AU) | t1/2 (min) | Kd (nM) | Bmax (AU) | t1/2 (min) | |
| Hb | 555 | 42 | 1.4 | 5 | 71 | 186 | 11 | 57 | 1.8 |
| 1-1 HbHp | 119 | 23 | 2.3 | 39 | 37 | 442 | 31 | 37 | 2.3 |
