Table 2.
Primers used for MLVA in this study
|
Marker |
Primer sequence
a
|
Primer concentration |
|
|---|---|---|---|
| Forward | Reverse | ||
| fnVNTR-1 |
V-CAGCTAGAAGCTTATTCGCCTCTT |
CAGGGTAATGCCTTAACGCATAT |
0.2 μM |
| fnVNTR-2 |
F-GAGTATTCCCTGCACCTACAATGAT |
TGTCATGCCTTTTTCTCTAGAGGAT |
0.2 μM |
| fnVNTR-3 |
N-CAAACTCTTTCACAAGAGGAAGCAT |
TCTGAACTCTGCTCTTTTCCCTCTA |
0.2 μM |
| fnVNTR-4 |
F-TTGTGCAAACACATCGATAGGAGAT |
AACTGCATCATCAGCATCTCTTCTA |
0.2 μM |
| fnVNTR-4b |
GTGCTTTTGCCTGTACCACCTT |
GCATCATTTGATACACCGTCCA |
NA |
| fnVNTR-5 |
N-CAATCACTCATCAACCACTAGCCAT |
TGCTGACTAATGGCTGACTGTAGTT |
0.3 μM |
| fnVNTR-5b | CTCATCAACCACTAGCCATCACAT | AAAGCCTGGGCTATCTAAATGCT | NA |
aFluorescent dyes are abbreviated as follows: F, FAM (6-carboxyfluorescein); N, NED; V, VIC. bsequencing primers; NA, not applicable.