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. 2013 Dec 2;2013:283649. doi: 10.1155/2013/283649

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Copyright © 2013 Kai Naumann et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Assessment of the stability of RGC100 in FCS, mouse serum, or human serum. RGC100 (1.6 μM) was incubated in 80% serum at 37°C and samples were analyzed on 12% native PAGE at indicated time points (h, hours and d, days). The corresponding untreated dsRNA (0 h) is shown as a reference. (a) Incubation of RGC100 in FCS up to 5 h. (b) Incubation of RGC100 in mouse serum up to 5 h. (c) Incubation of RGC100 in human serum up to 5 h. (d) Incubation of RGC100 in FCS up to 7 days. (e) Incubation of RGC100 in mouse serum up to 7 days. (f) Incubation of RGC100 in human serum up to 7 days. (g) Incubation of dsRNA (25 bp) used as a control in FCS for 5 hours. (e) Incubation of dsRNA (25 bp) used as a control in mouse serum for 5 hours. (f) Incubation of dsRNA (25 bp) used as a control in human serum for 5 hours.