Figure 3.

Activation of JAWS II DCs by RGC100 in a dose-dependent manner. (a), cytokine and chemokine profile of JAWS II DCs activated by RGC100. RGC100 was incubated with JAWS II DCs for 16 h at a concentration of 250 μg/mL. Secretion of cytokines was measured by ELISA. Negative control consists of supernatant of cells incubated in the absence of RGC100. Values of negative control have been subtracted from the values represented on the graph. Values shown are mean ± SEM of two independent measures. (b), Dose-dependent activation of JAWS II DCs by RGC100. RGC100 was incubated with cells for 16 h at the indicated concentrations. Secretion of IL-6 was measured by ELISA. Values shown are mean ± SEM of two independent measures. Negative control consists of supernatant of cells incubated in the absence of RGC100. Values of negative control have been subtracted from the values represented on the graph.