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. 2004 May;72(5):2635–2647. doi: 10.1128/IAI.72.5.2635-2647.2004

FIG. 2.

FIG. 2.

(A and B) Comparison of anti-SmCT-SOD antibody responses to hSOD under denaturing (A) and nondenaturing (B) conditions. The top gels are acrylamide gels of SmCT-SOD-GST, GST, BSA, SmGPX, and hSOD antigens stained with Coomassie blue. The middle panels are Western blots after incubation with rabbit anti-SmCT-SOD-GST IgG antibodies (1:2,500). Bottom gels are Western blots after incubation with mouse anti-SmCT-SOD-GST IgG antibodies (1:10,000). (C) ELISA results showing the mean reactivity (± standard error of the mean) of rabbit anti-SmCT-SOD-GST sera (1:500) to SmCT-SOD and hSOD antigens from three experiments. (D) ELISA results showing reactivities of mouse anti-hSOD sera against SmCT-SOD-GST, GST, and hSOD proteins. The line represents the cutoff value (mean OD value + 3 times the SD) of preimmune sera.