FIG. 3.

Lipolytic activities of recombinant E. coli containing L. pneumophila plaB. Culture supernatants (A) and cell lysates (B) of E. coli containing pBCKS or its derivative pKH192, or pUC18 or its derivative pKH190 or pKH194, were mixed with DPPG, DPPC, MPLPG, MPLPC, or 1-MPG. In some cases, culture supernatants or cell lysates were diluted prior to incubation with lipids as indicated. After a 2-h (A) or 50-min (B) incubation at 37°C, the release of FFA was quantified. Data are expressed as differences between the amount of FFA released by the culture supernatant or cell lysate and the amount released by uninoculated LB broth or Tris-HCl buffer, respectively. Results are means and standard deviations of duplicate cultures and are representative of three independent experiments.