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. 2004 May;72(5):3022–3030. doi: 10.1128/IAI.72.5.3022-3030.2004

FIG. 5.

FIG. 5.

Analysis of MSP1a proteins from different A. marginale isolates expressed in E. coli. Recombinant E. coli cell extracts were separated by SDS-PAGE, transferred to a nitrocellulose membrane, and reacted with anti-MSP1a MAb ANA15D2 (A) or stained with carbohydrate-specific periodate oxidation and biotin hydrazide conjugation (B). Arrowheads indicate the recombinant MSP1a protein bands. Numbers on the left indicate molecular masses in kilodaltons. Lane 1, negative E. coli control; lanes 2 to 7, protein extract of recombinant E. coli cells expressing MSP1a protein from A. marginale isolates from Virginia (lane 2), Oklahoma (lane 3), California (lane 4), St. Maries (lane 5), Texas (lane 6), and Okeechobee (lane 7).