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Chemical deglycosylation of MSP1a with TFMS. Native (lanes 1 to 3) and deglycosylated (lanes 4 to 6) MSP1a was transferred to a nitrocellulose membrane and reacted with anti-MSP1a MAb ANA15D2 (lanes 1 and 4), anti-MSP1a MAb AFOR2.2F1 (lanes 2 and 5), and anti-FLAG M2 MAb (lanes 3 and 6).
