TABLE 1.
Expression of phosphorylation of MAPK by monocytes after coculturing with C. albicans supernatantsa
| Protein | N | RD of MAPK
|
P value | |
|---|---|---|---|---|
| Monocytes | Monocytes plus supernatant | |||
| Phospho-ERK | 5 | 450 ± 93 | 790 ± 132 | 0.004 |
| Phospho-38 | 3 | 410 ± 27 | 370 ± 21 | 0.036 |
| Phospho-SAP | 3 | 540 ± 37 | 560 ± 36 | 0.641 |
a
Monocytes were cocultured with concentrated C. albicans supernatant fluid for 1 min at 37°C and then were lysed with lysis buffer. The lysates were resolved by Nu-PAGE followed by Western blotting. RDs. of phosphorylated ERK/p38/SAPK are expressed relative to the total ERK/p38/SAPK. Results are expressed as means ± standard errors of the means for N number of replicated experiments. P values were determined by a paired t test. P values of <0.05 are considered significant.