FIG. 3.

Ratios of specific to total IgA in intestinal lavage fluids of mice immunized i.g. with lactococci expressing TTFC (pTREX1-TTFC) or a control strain (pTREX1). Intestinal lavage fluids were collected from groups of six mice on days 6 (open bars), 20(striped bars), and 46 (shaded bars) post-initial treatment. The samples were tested by ELISA for TTFC-specific and total IgA. Bars, mean ratios; error bars, standard deviations. Asterisks indicate values significantly different from those for nonvaccinated controls (P < 0.05).