FIG. 6.

Proliferative responses of SPLC from groups of six mice immunized i.p. with lactococci expressing TTFC (pTREX1-TTFC), a control strain (pTREX1), or TT in FCA (a) and of MLNC from mice immunized i.g. with recombinant lactococci (b). Control nonvaccinated groups were also included. SPLC and MLNC were cultured with LP-TT (open bars), LP-U (striped bars), or formalin-fixed pTREX1-TTFC (solid bars) or pTREX1 (speckled bars) lactococci. Unstimulated cells were also cultured. Proliferation was determined by [³H]thymidine incorporation, and results were expressed as SI (ratios of counts from stimulated cells to counts from unstimulated cells). Bars, mean SI. Error bars, standard deviations.