Percentages of cytokine-positive events among CD4-positive LPL. Mice were immunized i.g. with lactococci expressing TTFC (pTREX1-TTFC) (solid bars) or a control strain (pTREX1) (striped bars). Control unvaccinated mice were also included (open bars). LPL were isolated from the small intestine on days 7 and 42, cultured, fixed, and stained for CD4 and the intracellular cytokines IL-4, IL-5, IL-10, and IFN-γ.