Table 2.

Strains and plasmids/cosmids used in this work

Strains/plasmids	Description	Reference
E. coli
DY380	∆(mrr–hsdRMS–mcrBC) mcrA recA1 λ cl857, ∆(cro–bioA)<>tet	[46]
ET12567/pUZ8002	dam-13::Tn9 dcm-6 hsdM; carries RK2 derivative with defective oriT for plasmid mobilization, Kanr	[45]
GM2929	dam-13::Tn9 dcm-6 hsdR2 recF143	M. Marinus, Univ. of Massachussetts Medical School
S. coelicolor A3(2)
M145	Prototrophic, SCP1- SCP2- Pgl+	[45]
J2401	M145 whiA::hyg	[15]
J2408	M145 ∆whiH::ermE	[15]
K300	M145 ∆SCO1774-1773::vph	This work
K301	M145 ∆SCO1773::vph	This work
K302	M145 ∆SCO3857::vph	This work
K303	M145 ∆SCO4157::aac(3)IV	This work
K316	M145 ∆SCO0934::aac(3)IV	This work
K317	M145 ∆SCO7449-7451::aac(3)IV	This work
K318	M145 ∆SCO1195-1196::Ωaac	This work
K319	M145 ∆SCO4421::Ωaac	This work
Plasmids/cosmids
pCR-BluntII	Cloning vector	Invitrogen
pIJ773	Source of apramycin resistance cassette, aac(3)IV, oriT	[47]
pIJ780	Source of viomycin resistance cassette, vph, oriT	[47]
pHP450Ωaac	Source of apramycin resistance cassette, Ωaac	[48]
pIJ2925	pUC-derived E. coli vector with a modified polylinker; bla	[49]
pOJ260	Mobilizable vector, no replication or integration in S. coelicolor, Aprar	[50]
pSET152	Mobilizable vector, integrates at ϕC31 attB site, Aprar	[50]
pIJ82	Derivative of pSET152, Hygr	Helen Kieser, JIC, Norwich, UK
pRT801	Mobilizable vector, integrates at ϕBT1 attB site, Aprar	[51]
pIJ6902	Expression vector, thiostrepton-inducible tipAp promoter, integrates at ϕC31 attB site, Aprar	[52]
pKF218	pRT801 containing SCO1775-1773 with part of upstream region	This work
pKF219	pOJ260 containing SCO1775-1773 with part of upstream region	This work
pKF278	pIJ82 containing SCO7449-7451 with part of upstream region	This work
pKF210	Vector for cloning promoters upstream reporter gene encoding mCherry, based on pIJ6902	This work
pKF212	Promoter region of SCO0934 translationally fused to mCherry	This work
pKF213	Promoter region of SCO1773 translationally fused to mCherry	This work
pKF214	Promoter region of SCO1774 translationally fused to mCherry	This work
pKF215	Promoter region of SCO3857 translationally fused to mCherry	This work
pKF216	Promoter region of SCO4157 translationally fused to mCherry	This work
pKF217	Promoter region of SCO4421 translationally fused to mCherry	This work
M10	Cosmid containing SCO0934a	[53]
I51	Cosmid containing SCO1773 and SCO1774a	[53]
H69	Cosmid containing SCO3857a	[53]
D84	Cosmid containing SCO4157a	[53]
6 F11	Cosmid containing SCO4421a	[53]
5C11	Cosmid containing SCO7449-7451a	[53]
G11A	Cosmid containing SCO1195-1196a	[53]

^aCosmid used to delete corresponding gene and to amplify promoter regions and regions used for complementation.